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Gene fusion detection in paediatric low-grade gliomas by NanoString nCounter technology

Test Code:

HT0212

Synonym(s):

 

Specimen Container

Specimen Requirement

1. Formalin-Fixed Paraffin-Embedded block or
2. 10x unstained sections and 1 corresponding H&E slide.

Tissue must be non-necrotic, non-cauterised, and must not have undergone acid-based decalcification. Improper storage conditions may additionally compromise nucleic acid integrity.

Specimen Storage / Transport

Method

 

Orderable as STAT?

No

Turn Around Time

7 working days

Reference Value(s)

Testing Laboratory Location

KKH

Laboratory

Molecular Histopathology

Contact Number

6394 1402

Day and Time Performed

Mon – Fri: 0800 hrs– 1700 hrs

Orderable on CPOE?

No

Downtime Form

Additional Information

Background:
A proportion of paediatric low-grade gliomas have gene fusions and duplication events. Identification of such gene fusions are important for diagnosis and may provide prognostic and predictive information.

Purpose of test:
This test identifies any of 31 specific gene fusions and one duplication event known to be present in paediatric low-grade gliomas using NanoString nCounter technology.

Specimen requirements:
10 unstained sections of tumour and a corresponding H&E-stained histological section, OR a paraffin block of tumour.
 
Turnaround time:
The test is batched and performed every fortnight. The test itself takes 3 working days to complete.

Caveats:
RNA integrity and concentration must meet assay requirements. Low tumour content may result in an inaccurate result. The specific gene fusion present needs to be expressed at high levels for detection.

Proficiency testing:
Exchange with an overseas centre performing the same test.

Reference:
Ryall S. Multiplex Detection of Pediatric Low-Grade Glioma Signature Fusion Transcripts and Duplications Using the NanoString nCounter System. J Neuropathol Exp Neurol 2017; 76: 562–570.

Change History Notes

Others

Keywords

Gene fusion detection in paediatric low-grade gliomas by NanoString nCounter technology HT0212
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