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Medulloblastoma subgroup determination by NanoString nCounter gene expression profiling

Test Code:

HT0207

Synonym(s):

 

Specimen Container

Specimen Requirement

1. Formalin-Fixed Paraffin-Embedded block or
2. 10x unstained sections and 1 corresponding H&E slide.

Tissue must be non-necrotic, non-cauterised, and must not have undergone acid-based decalcification. Improper storage conditions may additionally compromise nucleic acid integrity.

Specimen Storage / Transport

Method

 

Orderable as STAT?

No

Turn Around Time

7 working days

Reference Value(s)

Testing Laboratory Location

KKH

Laboratory

Molecular Histopathology

Contact Number

6394 1402

Day and Time Performed

Mon – Fri: 0800 hrs– 1700 hrs

Orderable on CPOE?

No

Downtime Form

Additional Information

Background:
The 2016 World Health Organization Classification of Tumours of the Central Nervous System recognizes genetically-defined medulloblastoma subgroups – WNT-activated, SHH-activated and non-WNT/non-SHH (groups 3 and 4). Combined morphological and molecular information provides optimal prognostic and predictive information to guide clinical management.

Purpose of test:
The test assigns a medulloblastoma molecular subgroup on the basis of the expression level of 22 medulloblastoma signature genes using NanoString nCounter technology.

Specimen requirements:
10 unstained sections of tumour and a corresponding H&E-stained histological section, OR a paraffin block of tumour.

Turnaround time:
The test is batched and performed every fortnight. The test itself takes 3 working days to complete.

Caveats:
RNA integrity and concentration must meet assay requirements. Low tumour content may result in an inaccurate result. A small proportion of cases cannot be classified into any of the four subgroups.

Proficiency testing:
Exchange with an overseas centre performing the same test.

Reference:
Northcott PA et al. Rapid, reliable, and reproducible molecular sub-grouping of clinical medulloblastoma samples. Acta Neuropathologica 2012; 123: 615-626.

Change History Notes

Others

Keywords

Medulloblastoma subgroup determination by NanoString nCounter gene expression profiling HT0207
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