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Gene fusion detection in solid tumours by anchored multiplex PCR (Archer FusionPlex pan-solid assay)

Test Code:




Specimen Container

Specimen Requirement

1. Formalin-Fixed Paraffin-Embedded block or
2. 10x unstained sections and 1 corresponding H&E slide.

Tissue must be non-necrotic, non-cauterised, and must not have undergone acid-based decalcification. Improper storage conditions may additionally compromise nucleic acid integrity.

Specimen Storage / Transport



Orderable as STAT?


Turn Around Time

15 working days

Reference Value(s)

Testing Laboratory Location



Molecular Histopathology

Contact Number

6394 1402

Day and Time Performed

Mon – Fri: 0800 hrs– 1700 hrs

Orderable on CPOE?


Downtime Form

Additional Information

A proportion of solid tumours including sarcomas have gene fusions. Identification of specific gene fusions are important for diagnosis and may provide prognostic and predictive information.

Purpose of test:
This test identifies the presence of a gene fusion involving any of the 101 listed genes known to be involved in gene fusions in solid tumours of various histological subtypes by next-generation sequencing-based anchored multiplex PCR (Archer FusionPlex). Prior knowledge of the fusion breakpoints and partner genes is not required, and the breakpoints and partner genes are idenotified through their sequences.

Specimen requirements:
10 unstained sections of tumour and a corresponding H&E-stained histological section, OR a paraffin block of tumour.

Turnaround time:
The test is batched and performed every fortnight. The test itself takes 5 working days to complete.

RNA integrity and concentration must meet assay requirements. Low tumour content may result in an inaccurate result.

Proficiency testing:
College of American Pathologists proficiency testing programme.

Change History Notes



Gene fusion detection in solid tumours by anchored multiplex PCR (Archer FusionPlex pan-solid assay) HT0213

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