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MLH1 Methylation Analysis

Test Code:

HT0206

Synonym(s):

 

Specimen Container

Specimen Requirement

1. Formalin-Fixed Paraffin-Embedded block or
2. 10x unstained sections and 1 corresponding H&E slide.
 
Tissue must be non-necrotic, non-cauterised, and must not have undergone acid-based decalcification. Improper storage conditions may additionally compromise nucleic acid integrity.

Specimen Storage / Transport

Method

 

Orderable as STAT?

No

Turn Around Time

3 working days; Batch run every Monday

Reference Value(s)

Testing Laboratory Location

KKH

Laboratory

Molecular Histopathology

Contact Number

6394 1402

Day and Time Performed

Mon – Fri: 0800 hrs– 1700 hrs

Orderable on CPOE?

Yes

Downtime Form

Additional Information

Tumor testing by IHC and/or MSI analysis has been reported to generally detect abnormal DNA mismatch repair (MMR) protein expression in 15–25% of all patients with endometrial carcinomas. Subsequent to this, all MSI patients exhibiting loss of DNA MMR protein MLH1 expression can be further segregated using MLH1 gene methylation testing to identify those with somatic MLH1 promoter and those which are methylated at the MLH1 promoter, the latter requiring further testing with confirmatory germline mutational analysis.

Expected Test Result: Methylated; Unmethylated

The molecular test result must always be correlated with clinical and histopathological findings by an appropriately trained, qualified and experienced pathologist.
 
Quality Assurance: CAP (MSI)

Change History Notes

Others

Keywords

MLH1 Methylation Analysis HT0206
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