PAX3-FKHR t(2;13)(q35;q14) or PAX7-FKHR t(1;13)(p36;q14) Translocation (RT-PCR) (DISCONTINUED)

Specimen Container	-
Specimen Requirement	1. Formalin-Fixed Paraffin-Embedded block or 2. 10x unstained sections and 1 corresponding H&E slide. Tissue must be non-necrotic, non-cauterised, and must not have undergone acid-based decalcification. Improper storage conditions may additionally compromise nucleic acid integrity.
Orderable as Urgent?	No
Turn Around Time	7 Working days
Testing Laboratory Location	KKH
Laboratory	Molecular Histopathology
Contact Number	6394 1402
Day and Time Performed	Mon – Fri: 0800 hrs– 1700 hrs
Orderable on CPOE?	No
Downtime Form	Molecular Pathology
Additional Information	Alveolar rhabdomyosarcoma (ARMS) is one of two subtypes of the rhabdomyosarcoma family of soft tissue tumours. Genetic subtyping has identified PAX3-FKHR (2;13), PAX7-FKHR (1;13), and fusion-negative subsets of ARMS. Our real-time RT-PCR assay is capable of identifying both the PAX3-FKHR (2;13), and the PAX7-FKHR (1;13) translocations. Expected Test Result: PAX3-FKHR (2;13) and PAX7-FKHR (1;13) fusion is detected/ not detected (RNA integrity is acceptable as checked by housekeeping gene)/ The assay is inconclusive (RNA quality is not good enough) The molecular test result must always be correlated with clinical and histopathological findings by an appropriately trained, qualified and experienced pathologist. Quality Assurance: CAP (SARC) HT0201

Last Updated - 27 Jun 2025