Mycoplasma Hominis and Ureaplasma Species culture

Urethral swabs, prostatic secretions, semen, urinary calculi, cervical swabs, high vaginal swabs, endometrial tissue, tubal samples, fluid from the pouch of Douglas. Urine samples are not suitable.

 

Nasopharyngeal, throat and endotracheal secretions.

 

Swabs:

When swabs are obtained from patients, care should be taken to sample as many cells as possible, since mycoplasmas are cell-associated. As the swab-sticks may be toxic to the mycoplasmas, the swabs should be swirled and squeezed against the side of the container and then discarded. They should never be left in the container.

 

Small pieces of tissue (<5g) or small volumes of fluid/pas should be sent in the mycoplasma transport media to ensure viability of the organism. Large amounts of such samples may be sent in plain, sterile containers.

 

B. CSF samples: 

CSF to be collected in sterile container.

 

10 mL of blood obtained aseptically should be inoculated into an anaerobic blood culture bottle.

 

All samples should be despatched to the laboratory as soon as possible.