Mycoplasma Hominis and Ureaplasma Species culture

Test Code:

IM0162

Synonym(s):

Specimen Container

A. Mycoplasma transport media is required for urogenital, respiratory, swab, tissue, and fluid samples.

B. Sterile container is required for CSF samples.

C. Anaerobic blood culture bottle is required for blood sample.

(To be obtained from DPLM Reception, after office hour call extension 1383)

Specimen Requirement

A. Urogenital samples:

Urethral swabs, prostatic secretions, semen, urinary calculi, cervical swabs, high vaginal swabs, endometrial tissue, tubal samples, fluid from the pouch of Douglas. Urine samples are not suitable.

Respiratory samples:

Nasopharyngeal, throat and endotracheal secretions.

Swabs:

When swabs are obtained from patients, care should be taken to sample as many cells as possible, since mycoplasmas are cell-associated. As the swab-sticks may be toxic to the mycoplasmas, the swabs should be swirled and squeezed against the side of the container and then discarded. They should never be left in the container.

Tissue and fluid from all sites:

Small pieces of tissue (<5g) or small volumes of fluid/pas should be sent in the mycoplasma transport media to ensure viability of the organism. Large amounts of such samples may be sent in plain, sterile containers.

B. CSF samples:

CSF to be collected in sterile container.

C. Blood samples:

10 mL of blood obtained aseptically should be inoculated into an anaerobic blood culture bottle.

All samples should be despatched to the laboratory as soon as possible.

Specimen Storage / Transport

Refrigerate sample until transportation to SGH Lab. Do not freeze. Send sample in ice pack.

Method

Orderable as STAT?

Turn Around Time

3 to 11 days

Reference Value(s)

Testing Laboratory Location

SGH

Laboratory

Microbiology

Contact Number

6394 1352

Day and Time Performed

Mon - Fri: 0800 hrs to 1700 hrs
Sat: 0800 hrs - 1230 hrs

Orderable on CPOE?

Yes

Downtime Form

Bacteriology, Mycology,Parasitology Tests

Additional Information

Change History Notes

Others

Keywords

Mycoplasma Hominis and Ureaplasma Species culture IM0162

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