25-Hydroxyvitamin D (25OHD) [Vitamin D] | KK Women's and Children's Hospital

Specimen Container	Gel separator tube (Adult) Microtube (Paeds)
Specimen Requirement	Plain Blood Adult: 5 mL Paediatric: 1.3 mL Invert blood 5 times after blood collection ensures the clot-activating factors is well mixed with the blood. This will ensure results are reliable.
Orderable as Urgent?	No
Turn Around Time	1 to 4 days (Refer to date and time performed)
Reference Value(s)	Reference interval for all age groups: 20.0 - 100.0 ng/ml Vitamin D status is classified according to serum levels of 25-Hydroxyvitamin D as follows:- Deficiency : <12.0 ng/mL Insufficiency: 12.0 to <20.0 ng/mL Sufficiency: 20.0 to 100.0 ng/mL Potential toxicity: >100.0 ng/mL
Testing Laboratory Location	KKH
Laboratory	Clinical Chemistry
Contact Number	6394 1383
Day and Time Performed	Tuesday and Friday
Orderable on CPOE?	Yes
Downtime Form	Clinical Chemistry - Form A
Additional Information	New reagent formulation (Roche Elecsys Vitamin D total III) on 25th March 2022, no change in reference intervals

Change History Notes

27 May 2024 08:00 AM

Update of Specimen Requirement.
08 Dec 2021 01:00 PM

Updated Specimen Requirement, Day and Time Performed and Additional Information.

Last Updated - 19 Jun 2025