Archer FUSIONPlex pan solid tumour v2 assay

A proportion of solid tumours including sarcomas have gene fusions. Identification of specific gene fusions are important for diagnosis and may provide prognostic and predictive information.

Purpose of test:

This test identifies the presence of a gene fusion involving any of the 129 listed genes known to be involved in gene fusions in solid tumours of various histological subtypes by next-generation sequencing-based anchored multiplex PCR (Archer FusionPlex). Prior knowledge of the fusion breakpoints and partner genes is not required, and the breakpoints and partner genes are idenotified through their sequences. Refer to test request form for gene list.

RNA integrity and concentration must meet assay requirements. Low tumour content (<50%) may result in an inaccurate result.

Proficiency testing:

College of American Pathologists proficiency testing programme.