Hb Electrophoresis | KK Women's and Children's Hospital

Specimen Container

EDTA Tube

Specimen Requirement

Whole Blood

Adult: 3 mL blood

Paediatrics and Neonate: 500 uL blood

Gently invert the tubes several times to prevent clotting.

Specimen Storage/Transport

Send to laboratory as soon as possible after blood collection at room temperature.

Orderable as Urgent?

Turn Around Time

Routine: 1 week

Identification of abnormal variant may take longer than 1 week

Reference Value(s)

	Hb A (%)	Hb A2 (%)	Hb F (%)
0 - 9 months	No indicated reference intervals
* 9 - 15 months	Present	2.2 - 3.2	0.2 - 9.9
** 15 months - 2 years	Present	2.2 - 3.2	0.0 - 5.5
*** 2 years and above	Present	2.2 - 3.2	^# ≤ 1.0

There are no indicated reference intervals for patients 0 – 9 months.

* Reference intervals adopted from published poster presented at ISLH 2009.

Wong ECC, et al. Normal Pediatric Reference Intervals for Hemoglobins A, F, and A2 Using Capillary Zone Electrophoresis Methodology.

** Reference intervals adopted from published poster presented at ISLH 2009.

Wong ECC, et al. Normal Pediatric Reference Intervals for Hemoglobins A, F, and A2 Using Capillary Zone Electrophoresis Methodology.

Reference intervals validated by HA Lab in Yr 2023.

*** Reference intervals (Hb A and Hb A2) are adopted from Sebia, validated by HA Lab in Yr 2021.

^# Reference interval for Hb F adopted from Dacie and Lewis Practical Haematology 11^th Edition, validated by HA Lab Yr 2023.

Testing Laboratory Location

KKH

Laboratory

Haematology

Contact Number

6394 8120

Day and Time Performed

Mon – Fri: 0800 hrs– 1700 hrs

Orderable on CPOE?

Yes

Downtime Form

63170-Form-1080 Haematology Laboratory Downtime Form

Additional Information

Specimens sent outside the indicated hours will be processed and reviewed on the next working day.

The test process involves centrifugation which has a potential risk of aerosolisation. Haematologist approval is required to perform the test on highly infectious cases (confirmed / suspected).

Change History Notes

12 Sep 2024 04:00 PM

Updated Turn Around Time, Additional Information
01 Apr 2024 12:00 AM

Updated Reference Value(s).
01 Dec 2023 12:00 AM

Updated Reference Value(s).
06 Feb 2023 12:00 AM

Updated Specimen Storage / Transport, Reference Value(s), Contact Number, Day and Time Performed, Downtime Form, Additional Information.

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Last Updated - 05 Apr 2025